Performance Issue Investigation

My application has a performance problem:

SYMPTOM: [Slow response, high memory, CPU spike, etc.]

METRICS:
- Current: [measured performance]
- Expected: [target performance]
- Acceptable: [minimum acceptable]

WHEN IT OCCURS:
- [Specific conditions]
- Load level: [concurrent users, data volume, etc.]

ALREADY RULED OUT:
- [Things you've confirmed aren't the issue]

Please help me:
1. Identify likely bottleneck areas
2. Suggest profiling approaches
3. Provide optimization strategies to try